Covering 329km² of the Midland Valley, the PEDL 133 license covers the area around the company’s Grangemouth petrochemicals complex.
Dart Energy holds the remaining 49% share of the pre-exploration shale license.
Ineos Upstream CEO Gary Haywood said: "This is a logical next step for Ineos and we are very excited about it.
"We are one of very few businesses that can use shale gas as both a fuel and a petrochemical feedstock.
"With our large UK asset base, our existing capabilities in operating oil and gas facilities and our exemplary safety and environmental record, INEOS is well placed to become a major player in the UK onshore gas production sector."
The acquisition marks Ineos’ first foray into the shale exploration segment.
