About 34% people voted in favor of the power plant while 62.74% voted against it in a turnover of nearly 52%, according to Reuters.
The turnover was just over the threshold to make the referendum valid. However, this move does not mean end of the project, rather it leaves a question mark over its future.
Hitachi-GE Nuclear Energy, a Japanese-US joint venture, was in line to construct the proposed power plant.
Hitachi spokesperson Terry Kubo to the news agency that the company expected more debate on the Lithuanian plant as well as it was ready to provide all necessary information to the new government.
"Hitachi remains committed to contributing to the energy security of Lithuania with our technology, in response to its request," Kubo added.
The finance ministry anticipates €6.8bn in expenditure for building the plant. It also said that €4bn would come from loans, and the rest would be put up by investors.
Lithuania is one of the European Union states most dependent on imported energy.